Experiment_Enzymes

Investigating the effect of temperature and pH on enzyme action **Apparatus and materials: **
 * Title: **
 * 1) Bunsen burner, tripod stand and gauze
 * 2) 3 bater baths
 * 3) Thermometer
 * 4) 12 test tubes and rack
 * 5) Measuring cylinder
 * 6) 4 230 ml beakers
 * 7) 2 white tiles
 * 8) 2 droppers
 * 9) Ice cubes
 * 10)  Distilled water
 * 11)  Test tube holder
 * 12)  Stop watch
 * 13)  Goggles
 * 14) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> 2% starch solution
 * 15) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> 1% amylase solution
 * 16) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> 1 M hydrochloric acid solution
 * 17) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> 1 M Ammonium Hydroxide solution
 * 18) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> Iodine solution

<span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Procedure:

<span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Part A:
 * 1) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Label 8 test tubes A1, A2, B1, B2, C1, C2, D1, D2.
 * 2) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Add 5 cm <span style="font-family: Arial,Helvetica,sans-serif; font: normal normal normal 6.7px/normal Helvetica; letter-spacing: 0px;">3 <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">of start solution to tubes A1, B1, C1 and D1. Add 3 cm <span style="font-family: Arial,Helvetica,sans-serif; font: normal normal normal 6.7px/normal Helvetica; letter-spacing: 0px;">3 <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> of distilled water to D2 and then 3 cm <span style="font-family: Arial,Helvetica,sans-serif; font: normal normal normal 6.7px/normal Helvetica; letter-spacing: 0px;">3 <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">of amylase solution into tubes A2, B2 and C2.
 * 3) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Place A1 and A2 into a beaker of ice water, B1 and V2 into a water bath at 37°C.
 * 4) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Place C1 and C2 in boiling water.
 * 5) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Leave the tubes for 10 minutes to allow the solutions in the tubes to reach the temperature of the water baths.
 * 6) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Pour the contents of A2, B2, C2 and D2 into the starch tube, A1, B1, C1 and D1. Record the time immediately. Keep the tubes A1, B1, C1 and D1, containing the mixture in the respective water bath throughout the experiment and ensure that the temperatures are maintained.
 * 7) <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">After 10 minutes, test the mixture in each test tube with iodine solutions, on a white tile. Draw a table with appropriate headings and record the results and conclusions.

<span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;">Part B: 1. To each of the 4 test tubes, W, X, Y, Z, add 2 cm <span style="font-family: Arial,Helvetica,sans-serif; font: normal normal normal 6.7px/normal Helvetica; letter-spacing: 0px;">3 <span style="font-family: Arial,Helvetica,sans-serif; letter-spacing: 0px;"> of starch solution. <span style="font-family: Helvetica,Helvetica,sans-serif; font-size: 10px; line-height: normal;">Place the tubes in a water bath at 37°C. 2. Make up the contents of the 4 test tubes as shown in the table below 3. Test the solution with iodine solution at the beginning of the experiment and after 10 minutes. 4. Record your observations and conclusions.